Carbohydrate Polymers 87, 405-410. Harding, Stephen E. (2005). Polysaccharides-based nanoparticles as drug delivery systems. Qi P, Caoa M, Songa L, Chena C, Liua M, Li N, Wua D, Penga J, Hub G, Zhao J. This universalization of the parameters leads to a higher error ratio in the calculation of the respective molecular weights. Carbohydrate Polymers, 79, 380-390. Dynamic light scattering study confirmed the mono-disperse nature of dextran with hydrodynamic radius of 900 nm. Intrinsic viscosity determination offers a simple and useful method that requires low cost equipment and yields useful information on soluble macromolecules. The optical image and histological analysis confirmed that the micropores on the skin were recovered over time. Chen Rong Huei; Min Lang Tsai (1998). Aqueous solutions of amphiphilic polysaccharides: Concentration and temperature effect on viscosity. A sugar cane native dextran as an innovative functional excipient for the development of pharmaceutical tablets. Conducting a comparative analysis of the data shown in Table 1 can observer that parameter data Mark-Houwink a, obtained by these authors are very similar to that obtained in this work, which affirms its validity. Dulbeccos Phosphate-Buffered Saline (DPBS) was purchased from Welgene (Gyeongsan, Korea). Epub 2021 Feb 9. Advanced Drug Delivery Reviews, 60, 1650-1662. Kirkland, J. J.; Dilks, C. H.; Rementer, S. W. (1992). 10K, and c M.W. The effectiveness of the RF microporation technique in transdermal drug delivery of macromolecules and hydrophilic agents such as peptides, hormones, and vaccines has been demonstrated extensively in previous studies [16,17,18]. The human and pig skin permeability of FITC-dextran (M.W. HHS Vulnerability Disclosure, Help ; Bataille, B. Learn More. In this study, we investigated the effectiveness of RF microporation technology in vitro, by evaluating the permeation of fluorescent-conjugated dextran (FITC-dextran) of various molecular weights through the human and pig skin after microporation, using a transdermal diffusion cell system. TEWL measurements were taken 30min after microporation of the human and pig skin. (2003). The characteristic Rg/Rh value for a globular protein is ~0.775, which means that Rg is smaller than Rh. 2012;29(7):201729. Anal. 2013 May;64(3):346-54. doi: 10.3109/09637486.2012.734289. 2014 Sep;5(9):2324-30. doi: 10.1039/c4fo00319e. 2008;26(11):12618. The cumulative permeation of FITC-dextran 10K was 4.820.32M, in the human skin and 5.920.46M in the pig skin. Proc of SPIE. Careers. Kloster, C.; Bica C.; Rochas C.; Samios, D.; Geissler, E. (2000). In fact, short chains do contain minor branching points. Microporation was performed 2, 5, and 10 times on each sample. Amersham Biosciences, AA Edition, www.amershambiosciences.com. The column was eluted with bidestilled water a flow rate of 0.8 ml/min, injection 5L, back pressure 200psi. (1998). If there are particles in the sample, the incident laser light gets scattered in all directions. Polymolecularity and polydispersity in Molecular weight determinations. Carbohydrate Research, 340, 811-826. Peptide and protein transdermal drug delivery. 43-1, 115-147. The temperature increment can induce a macromolecule compaction (decreases in RH and []), generating by this way, a greater difficult to flowing due at requires an increase of energy consumption (Eavf<). In order to confirm the molecular weight the gel permeation chromatography (GPC) for standard dextrans is used in a range of molecular weights from 8.8 to 2000kDa and these correspond to the following equation. TEWL measurements were taken to assess the integrity of the skin barrier and the extent of SC disruption. Crini, G. (2005). Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. Studies carried out at different ionic strength you can see them in the references [64, 65] and works with different solvents in the references [66, 67]. 2018 Jan;184(1):1-11. doi: 10.1007/s12010-017-2522-4. The hydrodynamic radius of 2-NBDG glucose was too small to measure. In vitro studies with human cadaver skin is a viable, ethical, and economical alternative for in vivo animal studies. Keywords: intrinsic viscosity, dextrans, Mark-Houwink parameters, hydrodynamic, temperature, Journal of Polymer and Biopolymer Physics Chemistry, 2013 1 (1),
Guner, Ali (1999). The same way, many of these authors emphasize the non-linear or hyperbranched of dextran molecular weight range from 9 to 2000kDa [3,5,8,16, 37,38]. Similar results are obtained in references [35, 36, 37], using a system of dextran / ethylene glycol, and dextran/dimethylsulfoxide, respectively. However, the currently available transdermal systems are limited by low drug permeability across the skin due to the lipophilic barrier function of the stratum corneum (SC), the outermost skin layer of the skin [3,4,5]; the methods favor low molecular mass, and lipophilic molecules, making it difficult to exploit the transdermal route for large and water-soluble molecules such as proteins and peptides. By using this website, you agree to our European Polymer Journal, 42, 1086-1092. Progress in Measurement, Interpretation and Application to Structure in Dilute Solution. J. Santoro MM, Gaudino G. Cellular and molecular facets of keratinocyte reepithelization during. Biomaterials, 22, 1891-1898. However, the results did not show significant regeneration of micropore compared to previous in vivo studies which reported significant regeneration [25]. ; Aberle, T; Burchard, W. (2000). For data requests, please contact the authors. Journal of American Chemical Society, 76 (20), 5041-5052. CAS Macromolecules, 31, 7712-7716. Static and Dynamic Light-Scattering Solution Behavior of Pullulan and Dextran in Comparison. Garcia de la Torre, J.; Carrasco, B. Durand, A. Hydrodynamic radii ( RH) were determined by dynamic light scattering. 2015;32(5):170413. Article In the future, we plan to investigate the possibility of enhancing the delivery of other macromolecular drugs such as peptides and siRNAs using the RF-based microporation device. In addition, microporated pig skin permeated larger amounts of FITC-dextran than human skin, this can be attributed to the differences in structure between the human and pig skin; the pig skin layer is thinner (SC, 813m) than the human skin (SC, 1017m) and the pig hair follicle is longer (3871m) than the human hair follicle (18m). Granath, K. A. Part of The molecular mass of dextran as determined by the gel filtration and copper bicinchoninate method was approximately, 800 kDa. Increased TEWL values are evidence of micropores formation. A similar article of dextran/water [35] clearly demonstrating the dependence of Mark-Houwink parameters with temperature. Mitragotri S. Mechanical disruption of skin barrier for vaccine delivery. Huang S, Huang G. Preparation and drug delivery of dextran-drug complex. The [] is connected to the dimensions of the biopolymer molecule isolated in a certain solvent. ; Ortega, A.; Garcia de la Torre, J.; Harding, S.E. Drug Discov Today Technol. The micropores of the human cadaver skin were regenerated according to the time. High Manufacturing Standards of Molecular Probes Dextrans. (1990). Structural characterization of a new dextran with a low degree of branching produced by Leuconostoc mesenteroides FT045B dextransucrase. The human and pig skin samples were placed in the receiver chamber of the Transdermal diffusion cell system with the SC facing up and the donor chamber was fixed in place. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. Seven dextrans, provided by Sigma, with molecular weight of 8.8, 40, 71.9, 110, 200, 580 and 2000 kDa. International Journal of Biological Macromolecules, 23, 135-141. Karmarkar, S.; Garber, R.: Kluza, J.; Koberda, M. (2006). The exponent a is a function of polymer geometry, and varies from 0.5 to 2 and exponent is dimensionless. Each microporated sample was incubated in the skin culture medium. Mark-Houwink-Kuhn-Sakurada Exponent at the Condition. Each skin pores was captured using a microscope and the obtained images were analyzed using ImageJ software (National Institutes of Health, Bethesda, USA). Ahn, G.Y., Eo, HS., Kim, D. et al. It has been found that, when applied to water treatment, it can effectively act on refractory organic matter in wastewater through thermal decomposition and water molecular action to produce free Sung-Wook Choi. The Transdermal diffusion cell system (DHC-6TD, LOGAN instrument, USA) was used to assess FITC-dextran permeability in vitro. Springer Editions. Chen Rong Huei; Wei Yu Chen; Shang Ta Wang; Chu Hsi Hsu; Min Lang Tsai (2009). WebDextran displays excellent biocompatibility and have been used clinically for over 50 years. Quintanilla-Pineda M, Achou CG, Daz J, Gutirrez-Falcon A, Bravo M, Herrera-Muoz JI, Pea-Navarro N, Alvarado C, Ibaez FC, Marzo F. Foods. Ioan, C.E. ; Weisz, J. Masuelli, Martin A. Hydrodynamic properties of whole arabic gum. Epub 2012 May 28. 2023 BioMed Central Ltd unless otherwise stated. Hi Sir Alexander Malm , Thank you very much! Really appreciate your help! Ortega, A.; Garca de la Torre, J. Thus, typically, the dextran glomerular for a molecule with a Stokes-Einstein (SE) radius of 30 is approximately sevenfold higher than that of neutral horseradish Various average molecular weights (Mv, Mn, Mw, and Mz) instead of Mv were substituted in M-H equation. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. The Mark-Houwink parameters indicate the dependence with temperature (T) in the range from 20 to 50C, ie with increasing T a increases and kM-H decreases. Biomacromolecules 8, 2464-2475. Intrinsic viscosity of bovine serum albumin conformers. The explanation for the anomalous values of a and kM-H is because the mixture solute-solvent is highly compatible due a great compaction of biopolymer, from undisturbed state in the absence of interactions, where free energy of mixing less than zero, and emphasize the nature non-linear or hyperbranched of dextran for Mw high to 200kDa. Red blood cell rouleaux formation in dextran solution: dependence on polymer conformation. Data File Dextran. The molecular mass of dextran as determined by the gel filtration and copper bicinchoninate method was approximately, 800 kDa. This study demonstrated that hydrodynamic volume and radius of gyration of a dextran sample with M w < 20 kDa and its linear counterpart with equal M w are almost The biological activity of cationic liposomes in drug delivery and toxicity test in animal models. Format), Citation-(EndNote
Kasaai, Mohammad R. (2007). Federal government websites often end in .gov or .mil. The hydrodynamic 57 radius of the dextranase is 7 Unperturbed dimensions and the theta temperature of dextran in ethylene glycol solutions. Table 2 compares the properties of nanoparticles coated with one or other polymer.